RNA and protein synthesis requirements for the resumption of meiosis in rabbit oocytes: the role of cumulus cells.

نویسندگان

  • J Motlík
  • J Fulka
  • R Procházka
  • Z Rimkevicová
  • M Kubelka
  • J Fulka
چکیده

In vitro maturation of rabbit cumulus-enclosed oocytes was fully inhibited in alpha-amanitin- (100 micrograms/ml) and cycloheximide- (5 micrograms/ml) supplemented media. The inhibition was reversible and substantially reduced by delaying the addition of alpha-amanitin (2h) or cycloheximide (3 h). In contrast, both drugs did not inhibit germinal vesicle breakdown in denuded oocytes. Co-culture of granulosa cells (1 x 10(6)/ml) with denuded oocytes did not substitute for an intact cumulus. The data presented here suggest that the resumption of meiosis in rabbit cumulus-enclosed oocytes is dependent upon early transcriptional and translational events which probably occur within the cumulus cells.

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عنوان ژورنال:
  • Reproduction, nutrition, development

دوره 29 5  شماره 

صفحات  -

تاریخ انتشار 1989